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Leibniz-Institut für Nutztierbiologie (FBN)

Forschungsbereich Molekularbiologie

Wilhelm-Stahl-Allee 2

D-18196 Dummerstorf

Dr. rer. nat. Rainer Fürbaß (Wissenschaftler), Veronica Schreiter (Technische Assistentin), Marika Nimz (Doktorandin), PD Dr. rer. nat. Jens Vanselow (AG Leiter), Maren Anders (Forschungsingenieurin), Marion Spitschak (Tierärztin)

Arbeitsgebiet

Wir untersuchen die räumlichen und zeitlichen Expressionsmuster und molekularen Regulationsmechanismen leistungsrelevanter Gene in Nutztieren und im Modelltier Maus. Die Erkenntnisse tragen zu einem besseren Verständnis grundlegender Mechanismen der Ausprägung züchtungsrelevanter Merkmale bei.

Gegenwärtige Forschungsthemen

• Expressionsmuster von Genen, die für Schlüsselenzyme der Steroidhormonsynthese während der Follikulogenese, Luteinisierung und Plazentation im Rind kodieren
• Molekulare Mechanismen (epigenetische Modulation, Protein/DNA Interaktion), die die Expression steroidogener Gene im Verlauf des Östruszyklus und der Trächtigkeit beim Rind regulieren
• Identifizierung und Charakterisierung von Schlüsselgenen der weiblichen Reproduktionsleistung durch differentielle Expressionsprofilierung von Hoch-Fruchtbarkeits-Mauslinien
• Expressionsprofilierung und Untersuchung epigenetischer Modifikation im Zusammenhang mit Stoffwechselprägung durch frühe Ernährung (nutritional programming) im Mausmodell und beim Schwein

Expertise

• Analyse der Genexpression durch quantitative Echtzeit PCR (real-time PCR)
• Anylse der Genexpression und –regulation in Gewebekulturmodellen (z.b. bovine Granulosazellen, Trophoblasten)
• Reportergenanalyse in Modellorganismen (transgene Zelllinien und transgene Mäuse)
• Promotoranalyse und Identifizierung cis- und trans-aktiver Elemente und Faktoren durch in-silico Analyse, Gelretardierung (EMSA), Chromatinimmunpräzipitation (ChIP) und Affinitätschromatographie
• Analyse epigenetischer Modulation (DNA Methylierung, Chromatinstruktur, Histonmodifikation)
• Differentielle Expressionsprofilierung über Microarray-Verfahren

Publikationen

Fürbass R, Tomek W, Vanselow J. 2010. Upstream Stimulating Factors 1 and 2 Enhance Transcription from the Placenta-Specific Promoter 1.1 of the Bovine Cyp19 Gene. BMC Molecular Biology 11:5.

Vanselow J, Spitschak M, Nimz M, Furbass R. 2010. DNA Methylation Is Not Involved in Preovulatory Down-Regulation of CYP11A1, HSD3B1, and CYP19A1 in Bovine Follicles But May Play a Role for Permanent Silencing of CYP19A1 in Large Granulosa Lutein Cells. Biol Reprod 82:289-98.

Nimz M, Spitschak M, Schneider F, Furbass R, Vanselow J. 2009. Down-regulation of genes encoding steroidogenic enzymes and hormone receptors in late preovulatory follicles of the cow coincides with an accumulation of intrafollicular steroids. Domest Anim Endocrinol 37:45-54.

Fürbass R, Selimyan R, Vanselow J. 2008. DNA methylation and chromatin accessibility of the proximal Cyp19 promoter region 1.5/2 correlate with expression levels in sheep placentomes. Mol Reprod Dev 75:1-7.

Tiemann U, Brüssow KP, Dänicke S, Vanselow J. 2008. Feeding of pregnant sows with mycotoxin contaminated diets has no effect on fetal and maternal hepatic transcription of genes of the insulin-like growth factor system. Food Additives and Contaminants 25:1365-73.

Vanselow J, Selimyan R, Fürbass R. 2008. DNA methylation of placenta-specific Cyp19 promoters of cattle and sheep. Exp Clin Endocrinol Diabetes 116:437-42.

Vanselow J, Nürnberg G, Koczan D, Langhammer M, Thiesen HJ, Reinsch N. 2008. Expression profiling of a high-fertility mouse line by microarray analysis and qPCR. BMC Genomics 9(1):307.

Vanselow J, Fürbass R, Tiemann U. 2008. Cultured bovine trophoblast cells differentially express genes encoding key steroid synthesis enzymes. Placenta 29:531-8.

Nausch N, Manteuffel G, Vanselow J. 2007. 0.2kb promoter sequence of the murine Cyp19 gene target beta-galactosidase expression to specific brain areas of transgenic mice. J Steroid Biochem Mol Biol 103:119-128.

M, Langhammer M, Schneider F, Renne U, Vanselow J. 2007. Two high-fertility mouse lines show differences in component fertility traits after long-term selection. Reproduction, Fertility and Development 19:815-821.

Tiemann U, Schneider F, Vanselow J, Tomek W. 2007. In vitro exposure of porcine granulosa cells to the phytoestrogens genistein and daidzein: Effects on the biosynthesis of reproductive steroid hormones. Reprod Toxicol 24:317-325.

Alm H, Brüssow KP, Torner H, Vanselow J, Tomek W, Dänicke S, Tiemann U. 2006. Influence of Fusarium-toxin contaminated feed on initial quality and meiotic competence of gilt oocytes. Reprod Toxicol 22:44-50.

Furbass R, Winter A, Fries R, Kuhn C. 2006. Alleles of the bovine DGAT1 variable number of tandem repeat associated with a milk fat QTL at chromosome 14 can stimulate gene expression. Physiol Genomics 25:116-120.

Pohland R, Lenz S, Vanselow J, Tomek W. 2006. Detection of gene expression and enzyme activity of Cytochrom P450 arom (aromatase) in preantral and early antral bovine follicles depending on culture conditions in vitro. Archiv fur Tierzucht-Archives of Animal Breeding 49:29-40.

Vanselow J, Yang W, Herrmann J, Zerbe H, Schuberth HJ, Petzl W, Tomek W, Seyfert HM. 2006. DNA-remethylation around a STAT5-binding enhancer in the alphaS1-casein promoter is associated with abrupt shutdown of alphaS1-casein synthesis during acute mastitis. J Mol Endocrinol 37:463-477.

Hamel M, Vanselow J, Nicola ES, Price CA. 2005. Androstenedione Increases Cytochrome P450 Aromatase Messenger Ribonucleic Acid Transcripts in Non-Luteinizing Bovine Granulosa Cells. Mol Reprod Dev 70:175-183.

Vanselow J, Pohland R, Fürbass R. 2005. Promoter 2 derived Cyp19 expression in bovine granulosa cells coincides with gene-specific DNA hypo-methylation. Mol Cell Endocrinol 233:57-64.

Lenz S, Pöhland R, Becker F, Vanselow J. 2004. Expression of the bovine Aromatase Cytochrome P450 gene (Cyp19) is primarily regulated by promoter 2 in bovine follicles and by promoter 1.1 in corpora lutea. Mol Reprod Dev 67:406-413.

Vanselow J, Fürbass R, Rehbock F, Klautschek G, Schwerin M. 2004. Cattle and Sheep Use Different Promoters to Direct the Expression of Aromatase Cytochrome P450 Encoding Gene, Cyp 19, during Pregnancy. Domestic Animal Endocrinology 27:99-114.

Walzel H, Brock J, Pohland R, Vanselow J, Tomek W, Schneider F, Tiemann U. 2004. Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro. Glycobiology 14:871-881.

Golovine K, Schwerin M, Vanselow J. 2003. Three different promoters control expression of the Aromatase Cytochrome P450 Gene (Cyp19) in mouse gonads and brain. Biol Reprod 68:978-984.

Tiemann U, Tomek W, Schneider F, Vanselow J. 2003. Effects of the mycotoxins alpha- and beta-zearalenol on regulation of progesterone synthesis in cultured granulosa cells from porcine ovaries. Reprod Toxicol 17:673-681.

Zsolnai A, Anton I, Fésüs L, Estonba A, Schwerin M, Vanselow J. 2002. Allele distributions of two novel SNPs within the sheep Cyp19 gene. Journal of Animal Breeding and Genetics 119:402-405.

Fürbass R, Said HM, Schwerin M, Vanselow J. 2001. Chromatin structure of the bovine Cyp19 promoter 1.1: DNaseI hypersensitive sites and DNA hypomethylation correlate with placental expression. Eur J Biochem 268:1222-1227.

Lottmann H, Vanselow J, Hessabi B, Walther R. 2001. The tet-on system in transgenic mice - inhibition of the mouse pdx-1-gene activity by antisense-RNA expression in pancreatic beta-cells. J Mol Med 79:321-328.

Rehfeldt C, Kuhn G, Vanselow J, Furbass R, Fiedler I, Nurnberg G, Clelland K, Stickland C, Ender K. 2001. Maternal treatment with somatotropin during early gestation affects basic events of myogenesis in pigs. Cell Tissue Res 306:429-440.

Vanselow J, Fürbass R, Zsolnai A, Kalbe C, Said HM, Schwerin M. 2001. Expression of the aromatase cytochrome P450 encoding gene in cattle and sheep. Journal of Steroid Biochemistry and Molecular Biology 79:279-288.

Fürbass R, Kalbe C, Zsolnai A, Schwerin M, Vanselow J. 2000. Expression of the aromatase encoding gene in cattle and sheep. Reproduction in Domestic Animals 35:172-173.

Kalbe C, Fürbass R, Schwerin M, Vanselow J. 2000. Cis-acting elements regulating the placenta-specific promoter of the bovine Cyp19 gene. Journal of Molecular Endocrinology 25:265-273.

Schmidt P, Kühn Ch, Kang´a S, Hanotte O, Vanselow J, Anton I, Langner Ch, Schwerin M. 2000. Interleukin-12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4. Anim Genet 31:283-285.

Vanselow J, Kühn Ch, Fürbass R, Schwerin M. 2000. Isolation of the bovine Cyp19 promoter 1.2 and identification of genetic variants. Anim Genet 31:337-338.

Goldammer T, Brunner RM, Vanselow J, Zsolnai A, Fürbass R, Schwerin M. 1999. Assignment of the bovine aromatase encoding gene CYP19 to 10q26 in goat and 7q24-q31 in sheep. Cytogenet Cell Genet 85:258-259.

Vanselow J, Zsolnai A, Fésüs L, Fürbass R, Schwerin M. 1999. Placenta-specific transcripts of the aromatase encoding gene include different untranslated first exons in sheep and cattle. Europ J Biochem 265:318-324.

Vanselow J, Kühn Ch, Fürbass R, Schwerin M. 1999. Three PCR/RFLPs identified in the promoter region 1.1 of the bovine aromatase gene (CYP19). Anim Genet 30:232-233.

Vanselow J, Zsolnai A, Fésüs L, Schmidt P, Schwerin M. 1999. A Bsp143I PCR-RFLP in exon 3 of the ovine aromatase gene (CYP19). Anim Genet 30:402-403.

Brunner RM, Goldammer T, Fürbass R, Vanselow J, Schwerin M. 1998. Genomic organization of the bovine aromatase encoding gene and a homologous pseudogene as revealed by DNA fiber FISH. Cytogenet Cell Genet 82:37-40.

Fürbass R, Kalbe C, Vanselow J. 1997. Tissue-specific expression of the bovine aromatase encoding gene uses multiple transcriptional start sites and alternative first exons. Endocrinology 138:2813-2819.

Rehfeldt C, Kuhn G, Kanitz E, Schneider F, Vanselow J, Fürbass R, Nürnberg G, Beyer M, Ender K. 1996. Fetal growth and skeletal muscle development in response to somatotropin treatment during early gestation. In: p 142.

Fürbass R, Vanselow J. 1995. An aromatase pseudogene is transcribed in the bovine placenta. g 154:287-292.

Schwerin M, Brockmann G, Vanselow J, Seyfert H-M. 1995. Perspectives of molecular genome analysis in livestock improvement - an overview. Animal Research and Development 42:14-26.

Vanselow J, Fürbass R. 1995. Novel aromatase transcripts from bovine placenta contain repeated sequence motifs. g 154:281-286.

Goldammer T, Guérin G, Brunner RM, Vanselow J, Fürbass R, Schwerin M. 1994. Chromosomal mapping of the bovine aromatase gene (cyp19) and an aromatase pseudogene to chromosome 10 and syntenic group U5. Mammalian Genome 5:822-823.